o:1616
Isolation and Characterization of Novel Canine Osteosarcoma Cell Lines from Chemotherapy-Naïve Patients
en
The present study aimed to establish novel canine osteosarcoma cell lines (COS3600, COS3600B, COS4074) and characterize the recently described COS4288 cells. The established D-17 cell line served as a reference. Analyzed cell lines differed notably in their biological characteristics. Calculated doubling times were between 22 h for COS3600B and 426 h for COS4074 cells. COS3600B and COS4288 cells produced visible colonies after anchorage-independent growth in soft agar. COS4288 cells were identified as cells with the highest migratory capacity. All cells displayed the ability to invade through an artificial basement membrane matrix. Immunohistochemical analyses revealed the mesenchymal origin of all COS cell lines as well as positive staining for the osteosarcoma-relevant proteins alkaline phosphatase and karyopherin α2. Expression of p53 was confirmed in all tested cell lines. Gene expression analyses of selected genes linked to cellular immune checkpoints (CD270, CD274, CD276), kinase activity (MET, ERBB2), and metastatic potential (MMP-2, MMP-9) as well as selected long non-coding RNA (MALAT1) and microRNAs (miR-9, miR-34a, miR-93) are provided. All tested cell lines were able to grow as multicellular spheroids. In all spheroids except COS4288, calcium deposition was detected by von Kossa staining. We believe that these new cell lines serve as useful biological models for future studies.
P53 Mutations; C-Met; Receptor Expression; Establishment; Tumor; Overexpression; Involvement
1552099
10.3390/cells12071026
2023-05-10T15:01:26.453Z
44
yes
46
Natascha
Leitner
University of Veterinary Medicine Vienna
Reinhard
Ertl
University of Veterinary Medicine Vienna
person
0000-0001-7485-3661
Simone
Gabner
University of Veterinary Medicine Vienna
person
0000-0001-8853-5939
Andrea
Fuchs-Baumgartinger
University of Veterinary Medicine Vienna
person
Ingrid
Walter
University of Veterinary Medicine Vienna
person
0000-0002-5038-188X
Juraj
Hlavaty
University of Veterinary Medicine Vienna
person
0000-0003-3050-5176
application/pdf
4068155
https://phaidra.vetmeduni.ac.at/o:1616
no
yes
16
70
1552253
Vaccines
22
12
7
MDPI
2023